Sunday, May 14, 2023 10:00 AM – 10:45 AM ET Exhibit Hall D, Room 1
Exhibitor Workshop: MBL International Corporation
Making Custom MHC Tetramers at the Lab Bench Using the QuickSwitch Kit
- Yuri Poluektov, Ph.D., Lab Scientist, Research and Development
In order to fully understand and characterize T cell populations, many MHCs with multiple different peptide combinations need to be screened and tested. A novel technology simplifies the process of screening numerous MHC-peptide combinations to simplify T cell analysis and make the assay cost efficient. In this workshop. we review procedures involved in using the QuickSwitch™ kit to make a custom MHC Tetramer that can be ready to stain T cells in 4 to 18 hours. We will discuss assay workflow and quantification of the binding affinity of the tested peptide for the MHC molecule using this kit.